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Higuchi, Mariko; Pinak, M.
no journal, ,
Ionizing radiation leads to clustered DNA damage sites, which are defined as two or more single lesions induced within 10-20 base pairs (bp). In general, these sites are repaired with less efficiency than single lesions. So far the detail mechanism of repair of multiple lesions is not known. A cluster damage containing a single strand break (SSB) within a few base pairs opposite to a 8-oxo-7,8-dihydroguanine (8oxoG) is one example of damage that is difficult to repair by hOGG1. In this study, we present results of molecular dynamics simulation of the models of complex of hOGG1 and cluster damaged DNA. Each model DNA contains SSB and 8oxoG. According to the estimated binding free energy, the affinity between hOGG1 and the DNA including SSB located -3bp from 8oxoG was smaller than that between hOGG1 and the DNA including SSB located +3bp from 8oxoG. This result supported the relation between inhibition effects by cluster damaged DNA and affinity between damaged DNA and hOGG1.
Matsumoto, Fumiko; Adachi, Motoyasu; Shimizu, Rumi; Meguro, Mizue; Tamada, Taro; Kato, Takashi; Kuroki, Ryota
no journal, ,
Thrombopoietin (TPO) is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets. The extracellular domain consists of 450 amino acid residues of thrombopoietin receptor (soluble TPO-R) contains two repeat of cytokine receptor homologous region, CRH-1 and CRH-2. In this work, we prepare CRH-1domein of TPO-R, and we discover that CRH-1 has binding site of TPO.
Tamada, Taro; Kinoshita, Takayoshi*; Yamada, Mitsugu; Kurihara, Kazuo; Ohara, Takashi; Adachi, Motoyasu; Tada, Toshiji*; Kuroki, Ryota
no journal, ,
no abstracts in English
Adachi, Motoyasu; Tamada, Taro; Kuroki, Ryota; Hidaka, Koshi*; Kimura, Toru*; Kiso, Yoshiaki*; Yamamoto, Kohei*; Kidokoro, Shunichi*
no journal, ,
no abstracts in English
-Lactamase derived from 
sp.560Arai, Shigeki; Yonezawa, Yasushi; Okazaki, Nobuo; Tamada, Taro; Tokunaga, Hiroko*; Ishibashi, Matsujiro*; Tokunaga, Masao*; Kuroki, Ryota
no journal, ,
We determined the crystal structure of halophilic -Lactamase obtained from sp.560 by X-ray crystallography. Since halophilic enzymes can bind many metal ions on its molecular surface, we are tring to find the Na
, Mg
and Cs binding sites of halophilic -Lactamase from determined crystal structure.
Arimori, Takao; Okazaki, Nobuo; Nakazawa, Masami*; Miyatake, Kazutaka*; Ueda, Mitsuhiro*; Tamada, Taro
no journal, ,
no abstracts in English
Shimizu, Rumi; Matsumoto, Fumiko; Arai, Shigeki; Ohara, Takashi; Adachi, Motoyasu; Tamada, Taro; Kuroki, Ryota; Nishimiya, Yoshiyuki*; Kondo, Hidemasa*; Tsuda, Sakae*
no journal, ,
no abstracts in English
-resolution data collection of beta-lactamase Toho-1 crystalKurihara, Kazuo; Tamada, Taro; Ohara, Takashi; Kuroki, Ryota
no journal, ,
no abstracts in English
Meguro, Mizue; Adachi, Motoyasu; Kuroki, Ryota; Tanizaki, Yuta*; Tahara, Ayaka*; Beppu, Miho*; Nagasawa, Kazumichi*; Kato, Takashi
no journal, ,
no abstracts in English